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Musculoskeletal diseases comprise the most frequent ailment for primary care physician visits in the United States, and the increases in incidence of musculoskeletal diseases with aging particularly osteoporosis and osteoarthritis is higher in women than in men, and leads to a significant amount of disability and reduced quality of life. Project 2 will test the hypothesis that chronic stress in IBS is associated with HPA axis dysregulation, increased visceral adipose tissue VAT accumulation, and circulating adipokines, which modulate HPA axis responsiveness, and mediate regional brain changes. It will prepare data for presentation, publication, subject safety analysis, and, eventually, public use.